There are 9 datasets available: 1. Picual all tissues 2. Picual stresses 3. Picual organs 4. Picual pollen tube growth 5. Picual seed 6. Picual cold stress 7. Picual wound stress 8. Picual Verticillium infection 9. Multiple varieties roots Verticillium infection -------------------------------------------------------------------------------- 1. Picual all tissues RNA-seq of roots, stem, meristem, leaves, flowers, pollen, fruits (epicarp + mesocarp), seeds, embryo, and testa+endosperm, combined from several experiments. Leaves and Roots are control samples from 4 month old potted plants in conditions as published by Leyva-Pérez et al., 2015. Seed, Embryo and Testa+endosperm are control samples in conditions as published by ??? et al., 2022. In preparation. Pollen samples correspond to mature pollen in conditions as published by Bullones et al., 2022. In preparation. The rest of the tissues come from three healthy 10-year-old olive trees under field conditions. They were published by Ramírez-Tejero et al., 2020. All samples were normalized to TPM. -------------------------------------------------------------------------------- 2. Picual stresses RNA-seq of leaves and roots from 4 month old potted olive plants in control and stess conditions. Abiotic stresses consisted of wounded roots and low temperatures, and biotic estress of infection with the fungal plant pathogen Verticillium dahliae. Verticillium infection was performed by root-dip inoculation in a conidia suspension of a Verticillium dahliae isolate representative of the so-called defoliating pathotype. Cold stress conditions were 10°C during the day and 4°C in the night, with 14 hours photoperiod. The plant samples employed for this study were obtained from a commercial nursery located in Córdoba province, Spain. Two biological replicates per sample were sequenced. cDNA libraries were sequenced by PE sequencing (100 x 2) with an Illumina HiSeq 1000 sequencer and normalized to TPM. This dataset was published by Leyva-Pérez et al. 2014 and raw data can be found in the BioProject PRJNA256033. -------------------------------------------------------------------------------- 3. Picual organs RNA-seq of roots, stem, meristem, leaves, flowers, and fruits (epicarp and mesocarp) of three healthy 10-year-old olive trees under field conditions. All the samples were collected at the same time, except for fruits, which had to be collected later upon reaching the turning ripening state. The plant samples employed for this study were obtained from the World Olive Germplasm Collection (WOGC) of the Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA). Two biological replicates per sample were sequenced, and each biological replicate consisted in an equilibrated pool of three plant RNAs. cDNA libraries were sequenced by PE sequencing (100 x 2) with an Illumina HiSeq 2000 sequencer and normalized to TPM. This dataset was published by Ramírez-Tejero et al., 2020 and raw data can be found in the BioProject PRJNA590386. -------------------------------------------------------------------------------- 4. Picual pollen tube growth RNA-seq of pollen tube growth at four times of development. Pollen grains were pre-hydratated by incubation in a humid chamber at room temperature for 1 hour and then transferred to Petri dishes containing germination medium. Petri dishes were maintained at room temperature in the dark and under gentle agitation. Pollen grains were sampled at 1, 3 and 6 hours after transfer to the in vitro germination medium. Mature pollen grains were collected during the anthesis of adult olive trees growing at Estación Experimental del Zaidín in Granada, Spain. Three biological replicates per sample were sequenced and normalized to TPM. This dataset will be published by Bullones et al., 2022 (In preparation). -------------------------------------------------------------------------------- 5. Picual seed RNA-seq of mature seeds sampled from ripe olives. Fruits were manually destoned and seeds were extracted by mechanical breakage of the woody endocarp. The seed tissues (testa + endosperm and embryo) were dissected with a scalpel. Olive fruits were collected 210 days after anthesis from adult olive trees growing at the Estación Experimental del Zaidín in Granada, Spain. One biological replicate of Seed and three biological replicates of Testa+Endosperm and Embryo were sequenced. Data shown were normalized to TPM. This dataset will be published by ??? et al., 2022 (In preparation). -------------------------------------------------------------------------------- 6. Picual cold stress RNA-seq of leaves from 4 month old potted olive plants in control and low temperature stess conditions, with 10°C during the day and 4°C in the night, with 14 hours photoperiod. The plant samples employed for this study were obtained from a commercial nursery located in Córdoba province, Spain. Two biological replicates per sample were sequenced. cDNA libraries were sequenced by PE sequencing (100 x 2) with an Illumina HiSeq 1000 sequencer and normalized to TPM. This dataset was published by Leyva-Pérez et al. 2014 and raw data can be found in the BioProject PRJNA256033. -------------------------------------------------------------------------------- 7. Picual wound stress RNA-seq of leaves and roots from 4 month old potted olive plants in control conditions and with wounded roots. The plant samples employed for this study were obtained from a commercial nursery located in Córdoba province, Spain. Two biological replicates per sample were sequenced. cDNA libraries were sequenced by PE sequencing (100 x 2) with an Illumina HiSeq 1000 sequencer and normalized to TPM. This dataset was published by Leyva-Pérez et al. 2014 and raw data can be found in the BioProject PRJNA256033. -------------------------------------------------------------------------------- 8. Picual Verticillium infection RNA-seq of leaves and roots from 4 month old potted olive plants in control conditions and infected with the fungal plant pathogen Verticillium dahliae. The infection was performed by root-dip inoculation in a conidia suspension of a Verticillium dahliae isolate representative of the so-called defoliating pathotype. The plant samples employed for this study were obtained from a commercial nursery located in Córdoba province, Spain. Two biological replicates per sample were sequenced. cDNA libraries were sequenced by PE sequencing (100 x 2) with an Illumina HiSeq 1000 sequencer and normalized to TPM. This dataset was published by Leyva-Pérez et al. 2014 and raw data can be found in the BioProject PRJNA256033. -------------------------------------------------------------------------------- 9. Multiple varieties roots Verticillium infection RNA-seq of roots from 14 to 20 years old olive plants corresponding to 36 olive cultivar in good phytosanitary conditions without any disease symptoms. The plant samples employed for this study were collected at the World Olive Germplasm Bank at IFAPA Centro 'Alameda del Obispo' located in Córdoba province, Spain. Two biological replicates per sample were sequenced. cDNA libraries were sequenced by PE sequencing (100 x 2) with an Illumina HiSeq 2500 sequencer and normalized to RPKM. This dataset was published by Ramírez-Tejero et al. 2021 and raw data can be found in the BioProject PRJNA638671.